DOI | Resolve DOI: https://doi.org/10.3892/ijo.2012.1721 |
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Author | Search for: Song, Kun; Search for: Xu, Peng1; Search for: Meng, Yongde1; Search for: Geng, Feng; Search for: Li, Jie2; Search for: Li, Zhao1; Search for: Xing, James; Search for: Chen, Jie1; Search for: Kong, Beihua |
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Affiliation | - National Research Council of Canada. Security and Disruptive Technologies
- National Research Council of Canada. Human Health Therapeutics
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Format | Text, Article |
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Subject | glucose capped gold nanoparticle; radiosensitizing agent; unclassified drug; antineoplastic activity; apoptosis; cancer radiotherapy; cell killing; cellular distribution; comparative study; cytoplasm; drug effect; drug uptake; endocytosis; flow cytometry; human cell; internalization; nick end labeling; radiosensitization; transmission electron microscopy; X irradiation |
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Abstract | The present study explored the cellular uptake dynamics, the subcellular location and the internalization mechanisms of gold nanoparticles (GNPs) and glucose-capped GNPs (Glu-GNPs). The cancer radiotherapy-enhancing effects of GNPs were also evaluated. We synthesized the GNPs and Glu-GNPs by the seeding technique. The effects on cellular uptake and the radiosensitizing effect induced by GNPs and Glu-GNPs at lower doses were investigated using two human cancer cell lines (HeLa and MCF-7). The intracellular location of the nanoparticles was analyzed by transmission electron microscopy (TEM). Analysis of cellular apoptosis following GNP-based radiotherapy was performed by flow cytometry and TUNEL assay. Cancer cells took up more Glu-GNPs than naked GNPs and the uptake curve showed size- and cell-dependent uptake. GNPs were mainly located in the cytoplasm and endocytosis is the mechanism behind the internalization of GNPs and Glu-GNPs. Lower doses of GNPs and Glu-GNPs still enhanced the killing effect using X-ray irradiation, although the apoptotic rate was not altered. The results presented in this study provide evidence that Glu-GNPs may have a bright future in tumor-targeted diagnosis and treatment. |
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Publication date | 2012-11-29 |
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In | |
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Language | English |
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Peer reviewed | Yes |
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NPARC number | 21270458 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | 9125de9d-04b0-49e1-b9f1-cf62b9b38f90 |
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Record created | 2014-02-12 |
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Record modified | 2020-04-21 |
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