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Evaluation of methods for preparing proteins from plasma high density lipoproteins

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1139/o71-157
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Affiliation
  1. National Research Council of Canada. Prairie Regional Laboratory
FormatText, Article
Abstract
The proteins from plasma high density lipoproteins have been prepared by three methods and the products are compared by electrophoresis on or in the media of paper, agarose, cellulose acetate, and polyacrylamide gel and by analytical ultracentrifugation. The capacities of the proteins to bind phospholipids, free fatty acids, and cholesterol are compared. The method based on that of Shore and Shore is the easiest to perform. The peptides are less likely to be denatured since no precipitation step is involved; this is supported by their generally greater affinity for lipids.
Publication date
PublisherCanadian Science Publishing
In
  • Canadian Journal of Biochemistry 49, no. 9 (1 September 1971): 1076–1082.
LanguageEnglish
Peer reviewedYes
IdentifierNRCC-12048
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Record identifier9366aada-9454-4dc1-966b-6f7a2bf14d1c
Record created2023-09-06
Record modified2023-09-06
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