| Author | Search for: Kartha, K. K.1; Search for: Gamborg, O. L.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Funder | Search for: International Development Research Centre (Canada); Search for: Centro Internacional de Agricultura Tropical |
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| Format | Text, Article |
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| Conference | The International Exchange and Testing of Cassava Germ Plasm: an Interdisciplinary Workshop held at CIAT, February 4-6, 1975, Palmira, Colombia |
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| Abstract | A procedure ha'i been developed for regenera~ tíng complete plants from shoot apícal meristems of cassáva. The method was used to ohtain symptom-free plants from stakes infecled with the cassava mosaie dísease of Indian and Nigerian origino Merislern tips cultured on a mineral salt-sucrose-vitamin medium SUPR1emenled witb a 5 x !O., M benzyladenine. 10' M naphthaleneacetic acid, !O" M gibberellic acid (OA,) regeneratOO complete plants wilhin 26 day.. More than 90"1. of the meristetn lips developOO into complete plants out of which 60010 were free of mosaic symptoms. In diseased stakes grown under higher temperature (350C) for 30 days (16h/day al 4000 lux light intensity and 70Ofo relative humidity), the mosaic symptom, completely disappeared, Symptoms reappeared when plants were transferred to a lower temper.ture (21°C). A11 plant' regeneratOO from meristems of symptom-free cassava were hea.lthy. Graft transrnission carrled out monthly confirmOO the aoseneo of symptoms of the causative agent in the plants regenerated by tissue culture techníques. |
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| Publication date | 1975 |
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| Publisher | CGIAR |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 14697 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 951aaf2b-0ccb-4cd5-a87a-26a1b42720c9 |
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| Record created | 2024-03-04 |
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| Record modified | 2024-03-04 |
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