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NMR spectroscopy and protein structure determination: Applications to drug discovery and development

From National Research Council Canada

Linkhttp://www.ncbi.nlm.nih.gov/pubmed/15853690
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FormatText, Article
Subjectnmr; protein; structure; spectroscopy; ligand; drug discovery; binding; Computer Simulation; Drug Design; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Protein Conformation; Protein Interaction Mapping; Proteins; Sequence Analysis, Protein
Abstract
Recent technological advances in NMR methods and instrumentation are having a significant impact in structural biology. These innovations are also impacting pharmaceutical biotechnology as it is now possible to use NMR spectroscopy to rapidly characterize a growing number of prospective protein drugs and protein drug targets. This review provides a general summary of how solution-state NMR can be used to determine protein structures. It also focuses on exploring how advances in solution state NMR are changing the way in which protein structures can be determined and protein-ligand interactions can be characterized. Recent innovations in protein sample preparation, in instrumentation and data collection, in spectral assignment and in structure generation are highlighted. The impact of solution-state NMR on pharmaceutical biotechnology is also discussed, with a special emphasis on describing how NMR has been used to study a number of pharmaceutically important proteins and how NMR is currently being used to rapidly screen and to map the binding sites of small molecules to a range of protein targets.
Publication date
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  • Current Pharmaceutical Biotechnology 6, no. 2 (April 2005): 105–20.
LanguageEnglish
Peer reviewedYes
NRC publication
This is a non-NRC publication

"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.

NRC number149
NPARC number8908561
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Record identifier95b61c5e-ce07-4eb9-82c9-a7d07a8e939c
Record created2009-04-22
Record modified2020-04-07
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