| DOI | Resolve DOI: https://doi.org/10.1016/S0304-3835(98)00036-6 |
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| Author | Search for: Liu, Kan-Zhi1; Search for: Schultz, Christian P.1; Search for: Mohammad, Ramzi M.; Search for: Al-Katib, Ayad M.; Search for: Johnston, James B.; Search for: Mantsch, Henry H.1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biodiagnostics
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| Format | Text, Article |
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| Subject | Chronic lymphocytic leukemia; Bryostatin 1; 2-Chlorodeoxyadenosine; Drug resistance; Infrared spectroscopy |
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| Abstract | Infrared (IR) spectroscopy was used to compare the drug resistance mechanism of cells from chronic lymphocytic leukemia (CLL) patients with that of WSU-CLL cells. Bryostatin 1 (Bryo 1), a macrocyclic lactone and protein kinase C activator, was used to render WSU-CLL cells more susceptible to 2-chlorodeoxyadenosine (2-CdA). The IR spectroscopic analysis revealed some changes in protein and DNA content in Bryo 1-treated WSU-CLL cells, however, the most significant alterations were observed in the membrane lipids, which resemble those found between 2-CdA-sensitive and 2-CdA-resistant cells from CLL patients. In addition, Bryo 1 treatment induced WSU-CLL cells to become CD11c, CD25 and tartrate-resistant acid phosphatase-positive, specific markers for hairy cell leukemia, a disease exquisitely sensitive to 2-CdA. Our results suggest that 2-CdA-sensitive CLL cells have cellular characteristics resembling the hairy cell stage. The similarity between the membrane lipids in 2-CdA-sensitive CLL cells and the Bryo 1-treated WSU-CLL cell line supports the suggestion that membrane lipid alteration might be an important step in the drug resistance mechanism of CLL cells. |
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| Publication date | 1998-05-15 |
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| Terms of use | - You may reproduce (print, make photocopies, or download) materials from NPArC without permission for non-commercial purposes (research, education, and private study), on the condition that you provide proper attribution of the sources in all copies.
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | S0304-3835(98)00036-6 |
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| NPARC number | 9201847 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 994b9d1c-aed5-46a0-b87e-189208238e97 |
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| Record created | 2009-10-03 |
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| Record modified | 2020-03-20 |
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