| DOI | Resolve DOI: https://doi.org/10.1007/s12195-013-0279-6 |
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| Author | Search for: He, Chuan; Search for: Gu, Quanrong; Search for: Zeng, Hongbo; Search for: Zhang, Hao; Search for: Huang, Min; Search for: Yang, Xiaoyan; Search for: Xing, James; Search for: Chen, Jie1 |
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| Affiliation | - National Research Council of Canada. Security and Disruptive Technologies
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| Format | Text, Article |
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| Subject | Cell membrane permeability; Microbubbles; DLVO force; Hydrodynamic force; High gravity field; Cavitation; THP-1 monocytes; MCF-7 cells |
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| Abstract | Cell permeability controls the transportation of extracellular materials through cell membranes, which plays a critical role in drug and gene delivery. This work reports an innovative method to enhance the cell permeability through cell-bubble interactions in a high gravity field. In the presence of microbubbles, the cell membrane permeability of mammalian cells was significantly increased in the high gravity field, and up to 80% THP-1 and 70% MCF-7 cells were permeabilized by using FITC-Dextran with average molecular weight of 40 and 70 kDa as fluorescent markers which were found to locate in both cytoplasm and cell nucleus by using a confocal microscope. Micro-scale pores were detected on the cell membrane by a scanning electron microscope after the cellmicrobubble interactions in the high gravity field. The delivery efficiency of FITC-Dextran could be further enhanced in gravity field of higher strength and in solutions with higher volume fraction of microbubbles, though the cell viability would also fall under extreme conditions. A simplified model was proposed to compare the contributions of surface forces (i.e., Derjaguin-Landau-Verwey-Overbeek (DLVO) interaction force and membrane undulation force) with hydrodynamic force associated with cell-bubble interactions in the high gravity field. The hydrodynamic force was found to dominate the cell-bubble interaction while the DLVO force and membrane undulation force only play an important role at small separation (≤10 nm) and low relative velocity of approach (i.e., low gravity field strength). The enhanced cell membrane permeability and formation of micro-scale pores are mainly due to the microbubble-cell interactions through collision and/ or cavitation effects from the bursting of microbubbles. Our results have important implications in many bioengineering processes which are dependent on cell membrane permeability. © 2013 Biomedical Engineering Society. |
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| Publication date | 2013-09-01 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21271790 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 9a97f3fa-eaf8-46e2-82a2-be22e5c1f25d |
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| Record created | 2014-04-22 |
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| Record modified | 2020-04-22 |
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