| Author | Search for: Michon, F.; Search for: Brisson, J.; Search for: Roy, R.; Search for: Ashton, F.; Search for: Jennings, Harold |
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| Format | Text, Article |
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| Subject | anisotropy; anomeric; antibody; antigen; antigenicity; bacterial; capsular polysaccharide; carbohydrate conformation; carbohydrate sequence; carbon; chromatography; chromatography, ion exchange; configuration; conformation; coupling; Fourier analysis; hydrolysis; immunology; isolation & purification; magnetic; magnetic resonance spectroscopy; magnetic-resonance; Neisseria; Neisseria meningitidis; NMR; NMR analysis; nuclear; nuclear magnetic resonance; one-bond; one-dimensional; polysaccharide; polysaccharides, bacterial; proton; resonance; structure; substituent; two-dimensional NMR |
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| Abstract | The capsular polysaccharide antigen of Neisseria meningitidis group I was isolated by Cetavlon precipitation and purified by ion-exchange chromatography. The structure of the I polysaccharide was determined largely by comprehensive proton and carbon-13 nuclear magnetic resonance studies in which both one-dimensional and two-dimensional experiments were carried out directly on the I polysaccharide. The I polysaccharide is composed of the repeating unit----4)alpha-L-GulpNAcA(1----3)[4-OAc]beta-D-ManpNA-cA(-- --in which the former residue adopts the 4C1 (L) conformation and the latter residue adopts the 4C1 (D) conformation. The one-bond coupling between the anomeric carbon and proton (1J13C,H) of the 2-acetamido-2-deoxy-beta-D-mannuronopyranosyl residue is not consistent with its beta-D configuration. This anomalous value of 1J13C,H for this residue is due to through-space anisotropy effects on its anomeric proton, generated by the proximity of the carboxyl group of the neighboring 2-acetamido-2-deoxy-alpha-L-guluronopyranosyl residue. The O-acetyl substituents of the I polysaccharide are essential for its antigenicity to group I polysaccharide-specific antibodies |
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| Publication date | 1985-09-24 |
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| In | |
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| Language | English |
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| NRC number | MICHON1985B |
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| NPARC number | 9358139 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 9c843270-13c1-466e-a0b4-4dbe19db654c |
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| Record created | 2009-07-10 |
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| Record modified | 2020-03-17 |
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