| Author | Search for: Gudlavalleti, S.; Search for: Szymanski, C.; Search for: Jarrell, Harold; Search for: Stephens, D. |
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| Format | Text, Article |
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| Subject | Acetylation; angle; Bacteria; capsular polysaccharide; carbohydrate; cell; CELL-SURFACE; complex; COMPLEXES; CPS; DISEASE; EXPRESSION; HIGH-RESOLUTION; Laboratories; magic angle; magic angle spinning; MUTANT; Neisseria; Neisseria meningitidis; NMR; NMR spectroscopy; NMR-spectroscopy; PATHOGENESIS; Polymers; POLYSACCHARIDE; SIGNALS; SPECTROSCOPY; STRAIN; structure; surface |
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| Abstract | High resolution-magic angle spinning (HRMAS) NMR spectroscopy was applied to serogroup A Neisseria meningitidis (NMA) to determine precise structures of capsular polysaccharide (CPS) expressed on the meningococcal surface. Both the O-acetylated (OAc) NMA parent and a mynC::aphA3 OAc- mutant demonstrated characteristic CPS-derived NMR signals indicating cell-surface expression of CPS, but only the parent expressed O-3 and O-4 acetylation signals. A capsule-defective strain showed no NMR signals for CPS. The (1)H NMR HRMAS spectral patterns correlated with the purified CPS (1)H NMR profiles. HRMAS NMR can distinguish detailed complex carbohydrate structures expressed on bacteria. NMA express both O-3 and O-4 acetylated polymers but not in equimolar ratio amounts in vivo |
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| Publication date | 2006-03-20 |
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| In | |
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| Language | English |
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| NRC number | GUDLAVALLETI2006 |
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| NPARC number | 9358579 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 9cf85dbe-f228-4eb6-9922-8d393d1890f5 |
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| Record created | 2009-07-10 |
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| Record modified | 2020-04-22 |
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