Toward reagent-free clinical analysis: quantitation of urine urea, creatinine, and total protein from the mid-infrared spectra of dried urine films

DOI	Resolve DOI: https://doi.org/10.1093/clinchem/46.9.1493
Author	Search for: Shaw, Anthony¹; Search for: Low-Ying, Sarah¹; Search for: Leroux, Michael; Search for: Mantsch, Henry H.¹
Affiliation	National Research Council Canada. NRC Institute for Biodiagnostics
Format	Text, Article
Abstract	Infrared (IR) spectroscopy offers an approach to clinical analysis that is conceptually very appealing. Whereas countless assays rely on the use of chemical agents to “recognize” the analyte of interest and to react with the analyte to produce specific color changes, IR-based analysis is founded on the rich IR absorption patterns that characterize the analytes themselves. These absorption patterns provide the basis to distinguish among the constituents and to separately quantify them. The most obvious distinguishing feature is that no reagents are required. In addition, IR-based analytical methods require very small sample volumes (typically microliters), show good precision over the entire physiological range, and are well suited for automation.
Publication date	2000-09-01
Publisher	Oxford University Press American Association for Clinical Chemistry
In	Clinical Chemistry 46, no. 9 (1 September 2000): 1493–1495.
Language	English
Peer reviewed	Yes
NRC number	1839
NPARC number	23000794
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Record identifier	9db0d4ef-00c7-4aab-844d-b400b7eeac67
Record created	2016-10-04
Record modified	2024-02-28

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