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Activity and utility of abscisic acid having a 3′ thioether linker arm

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/S0031-9422(96)00440-2
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Affiliation
  1. National Research Council of Canada. NRC Plant Biotechnology Institute
FormatText, Article
SubjectLepidium sativum; abscisic acid; linker arm; affinity chromatography; antibody; analogues; preparation; cress; germination
Abstract
Abscisic acid (ABA) was functionalized at the 3′-position by reaction of its α-2′,3′-epoxide with 1,4-butanedithiol to afford the corresponding derivative having a six-atom linker arm containing a terminal aulphhydryl group. This derivative was coupled with epoxy activated Sepharose 6B affording a gel containing ca 1.4 mg of (+)-ABA ml⁻¹. This gel was shown to bind specifically an anti-ABA antibody from hybridoma culture broth, and its binding capacity was determined. The (+)-ABA analogue possessing the thiol linker arm was also derivatized by reaction with a fluorescent derivatizing agent and these compounds were shown to inhibit cress seed germination.
Publication date
PublisherElsevier
In
  • Phytochemistry 44, no. 2 (January 1997): 215–220.
LanguageEnglish
Peer reviewedYes
IdentifierS0031942296004402
NRCC-40019
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Record identifiera31fce92-731a-43b1-ac27-ec00042fd961
Record created2023-11-02
Record modified2023-11-02
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