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Evaluation of media for simultaneous enumeration of total coliform and E.coli in drinking water supplies by membrane filtration techniques

From National Research Council Canada

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DOIResolve DOI: https://doi.org/10.1016/S1001-0742(08)60043-1
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Affiliation
  1. National Research Council of Canada. NRC Institute for Research in Construction
FormatText, Article
Subjectdifferential coliform agar (DCA); drinking water; E.coli; membrane filtration (MF); total coliform (TC); Water quality
Abstract
This study evaluated three different dehydrated media for simultaneous detection and enumeration of total coliform (TC) and E. coli in drinking water samples with a standard membrane filtration procedure. The experiment indicated that the differential coliform agar (DCA) medium was the most effective among the tested media in enumerating TC and E.coli, without the need for extensive accompanying confirmation tests. The results for DCA medium were highly reproducible for both TC and E.coli with standard deviation of 6.0% and 6.1%, respectively. A high agreement (82%) was found between DCA and m-Endo media on 152 drinking water samples in terms of TC positive. The DCA medium also reduced concealment of background bacteria.
Publication date
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  • Journal of Environmental Sciences 20, no. 3 (16 March 2008): 273–277.
LanguageEnglish
Peer reviewedYes
NRC numberNRCC 50037
NRC-IRC-18786
NPARC number20378152
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Record identifiera343987a-0cba-43b5-a9d2-610a0a36c86c
Record created2012-07-24
Record modified2020-04-15
Date modified: