| Download | - View accepted manuscript: HVOF-sprayed nano TiO₂-HA coatings exhibiting enhanced biocompatibility (PDF, 903 KiB)
|
|---|
| DOI | Resolve DOI: https://doi.org/10.1007/s11666-009-9442-y |
|---|
| Author | Search for: Lima, R. S.1; Search for: Dimitrievska, S.1; Search for: Bureau, M. N.1; Search for: Marple, B. R.1; Search for: Petiti, A.; Search for: Mwale, F.; Search for: Antoniou, J. |
|---|
| Affiliation | - National Research Council of Canada. NRC Industrial Materials Institute
|
|---|
| Format | Text, Article |
|---|
| Subject | Biocompatibility; biomedical coating; human mesenchymal stem cells (hMSCs); HVOF spraying; implants; nanostructured TiO₂ |
|---|
| Abstract | Biomedical thermal spray coatings produced via high-velocity oxy-fuel (HVOF) from nanostructured titania (n-TiO₂) and 10 wt.% hydroxyapatite (HA) (n-TiO₂-10wt.%HA) powders have been engineered as possible future alternatives to HA coatings deposited via air plasma spray (APS). This approach was chosen due to (i) the stability of TiO2 in the human body (i.e., no dissolution) and (ii) bond strength values on Ti-6Al-4V substrates more than two times higher than those of APS HA coatings. To explore the bioperformance of these novel materials and coatings, human mesenchymal stem cells (hMSCs) were cultured from 1 to 21 days on the surface of HVOF-sprayed n-TiO₂ and n-TiO₂-10 wt.%HA coatings. APS HA coatings and uncoated Ti-6Al-4V substrates were employed as controls. The profiles of the hMSCs were evaluated for (i) cellular proliferation, (ii) biochemical analysis of alkaline phosphatase (ALP) activity, (iii) cytoskeleton organization (fluorescent/confocal microscopy), and (iv) cell/substrate interaction via scanning electron microscopy (SEM). The biochemical analysis indicated that the hMSCs cultured on n-TiO₂-10 wt.%HA coatings exhibited superior levels of bioactivity than hMSCs cultured on APS HA and pure n-TiO2 coatings. The cytoskeleton organization demonstrated a higher degree of cellular proliferation on the HVOF-sprayed n-TiO₂-10wt.%HA coatings when compared to the control coatings. These results are considered promising for engineering improved performance in the next generation of thermally sprayed biomedical coatings. |
|---|
| Publication date | 2009-12-18 |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | No |
|---|
| NRC number | NRCC 52408 |
|---|
| NPARC number | 18398984 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | a39c67ce-f097-4211-bea7-784c8ad76161 |
|---|
| Record created | 2011-08-17 |
|---|
| Record modified | 2020-04-16 |
|---|
