| DOI | Resolve DOI: https://doi.org/10.1364/OE.21.004623 |
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| Author | Search for: Janz, S.1; Search for: Xu, D.-X.1; Search for: Vachon, M.1; Search for: Sabourin, N.1; Search for: Cheben, P.1ORCID identifier: https://orcid.org/0000-0003-4232-9130; Search for: McIntosh, H.1; Search for: Ding, H.1; Search for: Wang, S.1; Search for: Schmid, J.H.1; Search for: Delâge, A.1; Search for: Lapointe, J.1; Search for: Densmore, A.1; Search for: Ma, R.1; Search for: Sinclair, W.1; Search for: Logan, S.M.2; Search for: MacKenzie, R.2; Search for: Liu, Q.Y.2; Search for: Zhang, D.2; Search for: Lopinski, G.3; Search for: Mozenson, O.4; Search for: Gilmour, M.; Search for: Tabor, H. |
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| Affiliation | - National Research Council of Canada. NRC Institute for Microstructural Sciences
- National Research Council of Canada. Human Health Therapeutics
- National Research Council of Canada. Measurement Science and Standards
- National Research Council of Canada. Security and Disruptive Technologies
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| Format | Text, Article |
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| Subject | biosensor microarrays; chip layout; coupling light; delivery channels; fluid delivery; fully compatible; independent sensors; molecular binding; molecular biosensors; optical alignments; photonic wires; polyclonal antibody; probe molecules; real time; ring resonator; sensor chips; serotyping; silicon photonic wires; sub-wavelength; surface grating couplers; two-dimensional detectors; user input; bioassay; biochips; instruments; two dimensional; sensors; Escherichia coli |
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| Abstract | A complete photonic wire molecular biosensor microarray chip architecture and supporting instrumentation is described. Chip layouts with 16 and 128 independent sensors have been fabricated and tested, where each sensor can provide an independent molecular binding curve. Each sensor is 50 μm in diameter, and consists of a millimeter long silicon photonic wire waveguide folded into a spiral ring resonator. An array of 128 sensors occupies a 2 × 2 mm2 area on a 6 × 9 mm2 chip. Microfluidic sample delivery channels are fabricated monolithically on the chip. The size and layout of the sensor array is fully compatible with commercial spotting tools designed to independently functionalize fluorescence based biochips. The sensor chips are interrogated using an instrument that delivers sample fluid to the chip and is capable of acquiring up to 128 optical sensor outputs simultaneously and in real time. Coupling light from the sensor chip is accomplished through arrays of sub-wavelength surface grating couplers, and the signals are collected by a fixed two-dimensional detector array. The chip and instrument are designed so that connection of the fluid delivery system and optical alignment are automated, and can be completed in a few seconds with no active user input. This microarray system is used to demonstrate a multiplexed assay for serotyping E. coli bacteria using serospecific polyclonal antibody probe molecules. © 2013 Optical Society of America. |
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| Publication date | 2013 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21270711 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | a5b384ae-2e0c-4d41-8b8a-3027d39cc7ea |
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| Record created | 2014-02-17 |
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| Record modified | 2022-11-18 |
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