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Rhizosphere shotgun metagenomic analyses fail to show differences between ancestral and modern wheat genotypes grown under low fertilizer inputs

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1093/femsec/fiab071
AuthorSearch for: ORCID identifier: https://orcid.org/0000-0003-3408-0179; Search for: 1; Search for: 1; Search for: 2; Search for: ; Search for: ; Search for: ORCID identifier: https://orcid.org/0000-0002-7112-3425
Affiliation
  1. National Research Council of Canada. Energy, Mining and Environment
  2. National Research Council of Canada. Aquatic and Crop Resource Development
FormatText, Article
Subjectwheat; rhizosphere; shotgun metagenomics; nutrient cycling microbes; functional genes; low fertilization
Abstract
It is thought that modern wheat genotypes have lost their capacity to associate with soil microbes that would help them acquire nutrients from the soil. To test this hypothesis, ten ancestral and modern wheat genotypes were seeded in a field experiment under low fertilization conditions. The rhizosphere soil was collected, its DNA extracted and submitted to shotgun metagenomic sequencing. In contrast to our hypothesis, there was no significant difference in the global rhizosphere metagenomes of the different genotypes, and this held true when focusing the analyses on specific taxonomic or functional categories of genes. Some genes were significantly more abundant in the rhizosphere of one genotype or another, but they comprised only a small portion of the total genes identified and did not affect the global rhizosphere metagenomes. Our study shows for the first time that the rhizosphere metagenome of wheat is stable across a wide variety of genotypes when growing under nutrient poor conditions.
Publication date
PublisherOxford Academic
Federation of European Microbiological Societies
In
  • FEMS Microbiology Ecology 97, no. 6 (June 2021).
LanguageEnglish
Peer reviewedYes
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Record identifiera6887cd9-e6eb-4883-a17d-6eda26122f93
Record created2023-04-24
Record modified2023-04-24
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