| Download | - View accepted manuscript: Sensitive determination of domoic acid in mussel tissue using dansyl chloride derivatization and liquid chromatography-mass spectrometry (PDF, 1.4 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1039/c4ay01853b |
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| Author | Search for: Beach, Daniel G.1; Search for: Liu, Hechun1; Search for: Quilliam, Michael A.1 |
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| Affiliation | - National Research Council of Canada. Measurement Science and Standards
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| Format | Text, Article |
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| Abstract | This paper describes a new method for sensitive determination of domoic acid (DA), the causative toxin of amnesic shellfish poisoning (ASP), in shellfish. The method involves extraction of tissue homogenates with 50% methanol followed by a highly selective strong anion exchange solid phase extraction and a derivatization with dansyl chloride (DNS-Cl) to form the dansyl derivative of domoic acid (DNS-DA). Reaction times were very rapid and proceeded under ambient conditions to yield stable derivatives. A study of the collision-induced dissociation of ESI-produced protonated DNS-DA was carried out to identify the most sensitive transitions to use in development of a selected reaction monitoring detection method. Compared with un-derivatized DA, DNS-DA showed a 5-fold increase in sensitivity of MS/MS detection and improved retention on a reversed phase LC stationary phase. Resolution of DNS-DA and its isomers was achieved using isocratic elution in 15 min. |
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| Publication date | 2014-11-28 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21274935 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | a8711077-7874-44ce-bc0a-5c6692b4fbf2 |
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| Record created | 2015-05-01 |
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| Record modified | 2020-06-04 |
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