Elucidating the biochemical basis of trans‐16:1 fatty acid change in leaves during cold acclimation in wheat

Download

File	Format	Size
Final Version	PDF	1.7 MiB
Supplement 1	XLSX	182 KiB
Supplement 2	XLSX	14 KiB
Supplement 3	XLSX	9.6 MiB
Supplement 4	XLSX	201 KiB
Supplement 5	PDF	1.8 MiB

DOI

Resolve DOI: https://doi.org/10.1002/pei3.10044

Author

Search for: Li, Qiang¹ORCID identifier: https://orcid.org/0000-0003-0705-2850; Search for: Shen, Wenyun¹; Search for: Mavraganis, Ioannis¹; Search for: Wang, Liping¹; Search for: Gao, Peng¹ORCID identifier: https://orcid.org/0000-0002-6586-4307; Search for: Gao, Jie; Search for: Cram, Dustin¹; Search for: Li, Yifeng²; Search for: Liu, Ziying²; Search for: Fowler, David Brian; Search for: Pan, Youlian²ORCID identifier: https://orcid.org/0000-0002-0158-0081; Search for: Zou, Jitao¹

Affiliation

National Research Council of Canada. Aquatic and Crop Resource Development
National Research Council of Canada. Digital Technologies

Format

Text, Article

Subject

wheat; phosphatidylglycerol; trans‐16:1; cold acclimation

Abstract

In plant cells, phosphatidylglycerol (PG) in the chloroplast has a characteristic trans-∆3-hexadecenoic acid (t16:1) at the sn-2 position. The t16:1 content in wheat leaf tissues decreases during cold treatment, but the significance of this fatty acid compositional change and the underlying biochemical mechanism remains poorly understood. Using a large collection of wheat cultivars displaying a varying capacity of freezing tolerance, we show for the first time under field conditions that this low temperature induced t16:1 change is associated with winter hardiness. To explore the metabolic mechanism responsible for the reduction of t16:1, we performed detailed lipid analysis and comparative transcriptome study with four selected wheat lines under cold acclimation. Our results show that wheat leaf tissues experience a gradual decrease in chloroplast lipid pathway activity during cold acclimation and that the decline in chloroplast lipid synthesis manifests itself in the decrease of t16:1 in PG. Comparative RNA-seq analyses with leaf tissues further reveal concerted transcriptome shifts indicating a rebalancing of chloroplast and cytosolic lipid synthesis during cold acclimation. Our study, thus, provides mechanistic understanding on chloroplast lipid adjustments as a “molecular ideotype” and the t16:1 change as a specific metabolite marker for screening freezing tolerance in wheat.

Publication date

2021-05-17

Publisher

Wiley
New Phytologist Foundation

Licence

Creative Commons Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/

Language

English

Peer reviewed

Yes

NRC number

NRC-ACRD-PDB0121

Export citation

Export as RIS

Report a correction

Report a correction (opens in a new tab)

Record identifier

a92c9547-bf45-4911-a384-b9cc758bd6b0

Record created

2021-05-18

Record modified

2022-08-15

Date modified:: 2024-07-17