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Elucidating the biochemical basis of trans‐16:1 fatty acid change in leaves during cold acclimation in wheat

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DOIResolve DOI: https://doi.org/10.1002/pei3.10044
AuthorSearch for: 1ORCID identifier: https://orcid.org/0000-0003-0705-2850; Search for: 1; Search for: 1; Search for: 1; Search for: 1ORCID identifier: https://orcid.org/0000-0002-6586-4307; Search for: ; Search for: 1; Search for: 2; Search for: 2; Search for: ; Search for: 2ORCID identifier: https://orcid.org/0000-0002-0158-0081; Search for: 1
Affiliation
  1. National Research Council Canada. Aquatic and Crop Resource Development
  2. National Research Council Canada. Digital Technologies
FormatText, Article
Subjectwheat; phosphatidylglycerol; trans‐16:1; cold acclimation
Abstract
In plant cells, phosphatidylglycerol (PG) in the chloroplast has a characteristic trans-∆3-hexadecenoic acid (t16:1) at the sn-2 position. The t16:1 content in wheat leaf tissues decreases during cold treatment, but the significance of this fatty acid compositional change and the underlying biochemical mechanism remains poorly understood. Using a large collection of wheat cultivars displaying a varying capacity of freezing tolerance, we show for the first time under field conditions that this low temperature induced t16:1 change is associated with winter hardiness. To explore the metabolic mechanism responsible for the reduction of t16:1, we performed detailed lipid analysis and comparative transcriptome study with four selected wheat lines under cold acclimation. Our results show that wheat leaf tissues experience a gradual decrease in chloroplast lipid pathway activity during cold acclimation and that the decline in chloroplast lipid synthesis manifests itself in the decrease of t16:1 in PG. Comparative RNA-seq analyses with leaf tissues further reveal concerted transcriptome shifts indicating a rebalancing of chloroplast and cytosolic lipid synthesis during cold acclimation. Our study, thus, provides mechanistic understanding on chloroplast lipid adjustments as a “molecular ideotype” and the t16:1 change as a specific metabolite marker for screening freezing tolerance in wheat.
Publication date
PublisherWiley
New Phytologist Foundation
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  • Plant-Environment Interactions 2, no. 3, pei3.10044 (17 May 2021): 1–11.
LanguageEnglish
Peer reviewedYes
NRC numberNRC-ACRD-PDB0121
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Record identifiera92c9547-bf45-4911-a384-b9cc758bd6b0
Record created2021-05-18
Record modified2022-08-15

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