Language selection

Government of Canada / Gouvernement du Canada

Search

Electrochemical determination of naloxone using molecularly imprinted poly(para-phenylenediamine) sensor

From National Research Council Canada

Download
  1. (PDF, 1.6 MiB)
  2. (PDF, 194 KiB)
DOIResolve DOI: https://doi.org/10.1149/1945-7111/abbb0d
AuthorSearch for: 1; Search for: ; Search for: ; Search for: 1ORCID identifier: https://orcid.org/0000-0002-8205-7304
Affiliation
  1. National Research Council of Canada. Nanotechnology
FormatText, Article
Abstract
A molecularly imprinted polymer (MIP)-based electrochemical sensor featuring an electrochemically grafted para-phenylenediamine functional monomer on a reduced graphene oxide-gold nanoparticles composite modified screen printed electrode is reported. The morphology and properties of the sensing material were characterized with microscopy, spectroscopy and electrochemical techniques. A number of factors affecting the performance of the MIP sensor were examined and optimized. Under an optimized condition, the imprinted electrochemical sensor yielded homogenous naloxone binding sites with a dissociation constant of 8.6 μM, and responded linearly up to 8 μM naloxone, with a limit of detection of 0.16 μM. The sensor showed good run-to-run repeatability and batch-to-batch performance reproducibility with relative standard deviation of 5.7%–9.6% (n = 4) and <9% (n = 3), respectively. The imprinted sensor retained 95% and 85% of its performance when stored at ambient conditions for one and two weeks, respectively, demonstrating the sensor's good stability. Selectivity experiments showed that both the MIP sensor and non-imprinted polymer electrode had minimal response (<25%) to equal concentrations of structurally similar compounds such as morphine, naltrexone and noroxymorphone, indicating good selectivity of the MIP sensor towards naloxone. The MIP sensor was successfully used to quantify naloxone in artificial urine samples, yielding recoveries greater than 92%.
Publication date
PublisherIOP
The Electrochemical Society
Licence
In
  • Journal of The Electrochemical Society 167, no. 13, 137508: 1–9.
LanguageEnglish
Peer reviewedYes
NRC numberNRC-NANO-93
Export citationExport as RIS
Report a correctionReport a correction (opens in a new tab)
Record identifiera9b8c86c-4008-421e-8f57-aa608a9c184f
Record created2021-02-15
Record modified2021-02-15
Date modified: