A novel method for the quantification of bovine colostral immunoglobulin G using infrared spectroscopy

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.idairyj.2015.08.004
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Name affiliation
  1. National Research Council of Canada
FormatText, Article
Journal titleInternational Dairy Journal
ISSN0958-6946
Volume52
Pages35–41
SubjectAntigen-antibody reactions; Calibration; Infrared spectroscopy; Mammals; Analytical method; Calibration model; Immunodiffusion; Immunoglobulin G; Partial least-squares regression; PLS regression; Systematic bias; Test sets; Least squares approximations
Abstract
Transmission infrared (IR) spectroscopy, in combination with partial least squares (PLS) regression, was used as the basis to develop a new method to quantify IgG in bovine colostrum. Colostrum samples (n = 250) were tested simultaneously by the reference radial immunodiffusion (RID) assay and IR spectroscopy. Colostral IgG concentrations obtained by RID assay were linked to pre-processed spectra and divided into two sets, i.e., calibration and test. PLS regression was applied to the calibration set and calibration models were developed, and the test set was used to assess the accuracy of the analytical method. The Pearson and concordance correlations between test set IgG concentrations as determined by the IR assay and the RID assay were 0.91. The Bland-Altman plot showed no evidence of systematic bias between IR and RID methods. Transmission IR spectroscopy is an effective method for quantification of bovine colostral IgG concentration and for assessment of colostrum quality.
Publication date
PublisherElsevier
LanguageEnglish
Peer reviewedYes
NPARC number21277470
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