Real-time monitoring, diagnosis, and time-course analysis of microalgae Scenedesmus AMDD cultivation using dual excitation wavelength fluorometry

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/s10811-014-0494-0
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Name affiliation
  1. National Research Council of Canada. Measurement Science and Standards
  2. National Research Council of Canada. Aquatic and Crop Resource Development
  3. National Research Council of Canada. Energy, Mining and Environment
FormatText, Article
Journal titleJournal of Applied Phycology
ISSN0921-8971
Abstract
Dual excitation wavelength fluorometry was used for real-time monitoring and diagnosis of the growth of the microalgae Scenedesmus AMDD in a 300-L continuous photobioreactor (PBR). Emission spectra were acquired at 1-min intervals using excitation lights at 365 and 540 nm. Real-time dry weight estimations were achieved using linear regression with the chlorophyll peak, while protein estimations required a more complex Principal Component Regression model, which takes advantage of the full emission spectrum. The resulting regression coefficients were 0.95 and 0.80, respectively. Furthermore, the spectra were analyzed using multivariate curve resolution technique. The proposed approach for fluorescence-based, real-time measurements of key algae cultivation parameters and culture state diagnosis was successfully demonstrated in a 42-day PBR validation test.
Publication date
PublisherSpringer International Publishing
LanguageEnglish
Peer reviewedYes
NPARC number21275615
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Record created2015-07-14
Record modified2020-04-22
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