| DOI | Resolve DOI: https://doi.org/10.1139/b73-272 |
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| Author | Search for: Constabel, F.1; Search for: Kirkpatrick, J. W.1; Search for: Gamborg, O. L.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Abstract | Protoplasts isolated from mesophyll of Pisum sativum L. cv. Century, and cultured in 0.2-ml droplets of B5 medium with 1.0 mg/liter (2,4-dichlorophenoxy)acetic acid (2,4-D) or 1-naphthaleneacetic acid (NAA) and 2.0 mg/liter kinetin regenerated cell walls within 2–3 days. The resulting cells began to divide and form calli after 19 days of culture. Protoplast survival depended on keeping the leaf material in the dark for at least 30 h before use. |
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| Publication date | 1973-11-01 |
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| Publisher | Canadian Science Publishing |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | NRCC-13474 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | adea972e-49f5-46bc-bab1-af54e882c77e |
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| Record created | 2023-09-01 |
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| Record modified | 2023-09-01 |
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