| DOI | Resolve DOI: https://doi.org/10.1111/j.1399-3054.1974.tb03694.x |
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| Author | Search for: Kartha, K. K.1; Search for: Gamborgt, O. L.1; Search for: Constabel, F.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Abstract | Internodal segments from 6-weeks-old rape plants (Brassica napus L. cv. Zephyr) were induced to differentiate in vitro producing shoots or shoots and roots on synthetic nutrient medium under controlled conditions. Benzyladenine (BA) alone (5 × 10⁻⁶ M) induced multiple shoot formation on all stem explants. Roots were induced on shoots when recultured on nutrient medium supplemented with auxins such as naphthalene-acetic acid (NAA) or indoleacetic acid (1AA) or when planted in vermiculite. Complete plant formation was obtained when NAA (2 × 1⁻⁶, 5 × 10⁻⁶ and 10⁻⁵ M) was employed in conjunction with BA at 5 × 10⁻⁶M. At higher concentrations (10⁻⁵M) NAA retards the shoot development while 1AA suppresses it totally. Lower levels of auxins along with the cytokinin did not retard or inhibit shoot differentiation. |
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| Publication date | 1974-07 |
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| Publisher | Wiley |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | aec90a74-de35-498a-b7ad-6da0672dc1ef |
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| Record created | 2023-06-29 |
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| Record modified | 2023-07-03 |
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