DOI | Resolve DOI: https://doi.org/10.1007/978-1-4939-8730-6_1 |
---|
Author | Search for: L’Abbé, Denis1; Search for: Bisson, Louis1; Search for: Gervais, Christian1; Search for: Grazzini, Eric1; Search for: Durocher, Yves1 |
---|
Affiliation | - National Research Council of Canada. Human Health Therapeutics
|
---|
Format | Text, Book Chapter |
---|
Subject | transfection; 293-6E; polyethylenimine; deacylated PEI; recombinant protein; suspension culture; IMAC; protein-A; antibody; serum-free medium |
---|
Abstract | Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram quantities of r-proteins can be typically obtained within less than 10 days following transfection. In this chapter, we describe a simple and robust transfection process of suspension-growing human embryo kidney 293 cells using two commercially available serum-free media and polyethylenimine as the transfection reagent. This chapter provides examples for the production and purification of a his-tagged recombinant protein and two monoclonal antibodies. |
---|
Publication date | 2018-09-22 |
---|
Publisher | Springer |
---|
In | |
---|
Series | |
---|
Language | English |
---|
Peer reviewed | Yes |
---|
Export citation | Export as RIS |
---|
Report a correction | Report a correction (opens in a new tab) |
---|
Record identifier | b16e8413-e48e-4c23-93dd-71712cdffc2a |
---|
Record created | 2019-05-03 |
---|
Record modified | 2020-06-18 |
---|