| DOI | Resolve DOI: https://doi.org/10.1557/jmr.2012.323 |
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| Author | Search for: Parisien, A.; Search for: Al-Zarka, F.; Search for: Hussack, G.1; Search for: Baranova, E.A.; Search for: Thibault, J.; Search for: Qingdao Lan, C. |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | Adsorption cycle; Average diameter; Binding capacities; Ferromagnetic behaviors; magnetic; Magnetic adsorbents; Magnetic characterization; Magnetization-saturation; Modified polyol method; Nickel nanoparticles; Nucleating agents; Palladium chloride; Polyvinyl pyrrolidone; Protective agents; Protein purification; Single domains; Adsorbents; Adsorption; Alcohols; Amino acids; Antibodies; Chlorine compounds; Ethylene glycol; Magnetic domains; Nanomagnetics; Nanoparticles; Nickel; Nickel compounds; Palladium; Purification; Recombinant proteins; Saturation magnetization; X ray diffraction; Biosynthesis |
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| Abstract | Nickel nanoparticles (NNPs) synthesized by a modified polyol method using ethylene glycol as a reducing agent, palladium chloride as a nucleating agent, and polyvinylpyrrolidone (PVP) as a protective agent were investigated as a potential magnetic adsorbent for the purification of hexahistidine-tagged (His6-tagged) recombinant proteins. The synthesis resulted in nanoparticles having an average diameter of 6828 nm. The x-ray diffraction pattern confirmed the presence of nickel metal, as well as the presence of unreacted nickel (II) hydroxide Ni(OH)2. Magnetic characterization showed that a magnetization saturation of 39.3 electromagnetic unit (emu)/g at 20,000 Oersted (Oe) was reached rapidly and that the material exhibited ferromagnetic behavior. Protein purification results showed that the synthesized NNPs were highly selective for binding to a His6-tagged recombinant protein single-domain antibody ToxA5.1. In addition, NNPs were used for four adsorption cycles without significant binding capacity losses. These particles have shown great potential such as being easily synthesized, cost-effective, and highly selective magnetic adsorbents for the purification of His6-tagged recombinant proteins. © Copyright © Materials Research Society 2012. |
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| Publication date | 2012 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21269398 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | b1c3d0e3-befa-4853-86c1-742479160b0e |
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| Record created | 2013-12-12 |
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| Record modified | 2020-04-21 |
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