| Download | - View accepted manuscript: A sensitive electrochemical assay for early detection of HIV-1 protease using ferrocene-peptide conjugate/au nanoparticle/single walled carbon nanotube modified electrode (PDF, 877 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1080/00032711003653924 |
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| Author | Search for: Mahmoud, Khaled A.1; Search for: Luong, John H. T.1 |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | Electrochemistry; Ferrocene; Gold nanoparticle; HIV-1; Pepstatin; Protease; Single-walled carbon nanotube |
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| Abstract | A rapid and simple electrochemical detection method has been developed to detect the presence of protease (HIV-1 PR) in HIV patients' sera. In this assay format, disposable screen printed gold electrode (SPGE) surface was modified with gold nanoparticles (AuNPs) or thiolated single-walled carbon nanotubes/gold nanoparticles (SWCNT/AuNPs). Thiol-terminated ferrocene (Fc)-pepstatin was then self-assembled on such modified surfaces. The interaction between the Fc-pepstatin modified substrates and HIV-1 PR was confirmed by scanning electron (SEM) and atomic force microscopy (AFM) and probed by differential pulse voltammetry (DPV). A linear relationship was observed between the shift in peak potential and the increasing number of HIV-1 viral replications in the HIV-infected serum samples. |
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| Publication date | 2010 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 53331 |
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| NPARC number | 16152640 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | ba6993f8-34a6-4794-9c29-b41273f26f22 |
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| Record created | 2010-11-05 |
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| Record modified | 2020-04-17 |
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