| Download | - View accepted manuscript: Validation of a single-isotope-labeled cholesterol tracer approach for measuring human cholesterol absorption (PDF, 538 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1007/s11745-004-1206-6 |
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| Author | Search for: Wang, Yanwen1; Search for: Vanstone, Catherine A.; Search for: Parsons, William D.; Search for: Jones, Peter J. H. |
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| Affiliation | - National Research Council of Canada. NRC Institute for Nutrisciences and Health
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| Format | Text, Article |
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| ISSN | 0024-4201
1558-9307 |
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| Subject | aged; cross-over studies; fatty acids; female; ipoproteins, LDL/blood; lipoproteins, HDL/blood; triglycerides |
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| Abstract | Cholesterol absorption is frequently determined using the plasma dual stable-isotope ratio method (PDSIRM). However, this method involves intravenous injection of stableisotope-labeled cholesterol with simultaneous oral administration of differently labeled cholesterol, which results in high study costs and involves additional ethical considerations. The objective of the present study was to validate a simpler singleisotope method for determining cholesterol absorption against PDSIRM by using data from two previous studies. Enrichments of carbon-13 (¹³C) and deuterium in red blood cells were analyzed by using differential isotope ratio MS. The area under the curve of ¹³C-enrichment in the plasma free-cholesterol pool was found to be significantly correlated with cholesterol absorption measured by using PDSIRM for study 1 (r = 0.85, P < 0.0001) and study 2 (r = 0.81, P < 0.0001). Average ¹³C-enrichment correlated with the area under the curve of ¹³C-enrichment in the plasma free cholesterol for both study 1 (r = 0.98, P < 0.0001) and study 2 (r = 1.00, P < 0.0001). Study 1 examined the efficacy and mechanisms of unesterified plant sterols and stanols on lipid profiles in hypercholesterolemic men and women, while study 2 investigated the effects of phytosterol vs. phytostanol esters on plasma lipid levels and cholesterol kinetics in hyperlipidemic men. Experimental approaches to determine cholesterol absorption were identical between the two studies. Consequently, in both studies, correlations (r = 0.88, P < 0.0001 for study 1, and r = 0.82, P < 0.0001 for study 2) were found between the average ¹³C-enrichment of plasma free cholesterol and cholesterol absorption measured by PDSIRM. These results suggest that a single-isotope-labeled cholesterol tracer approach can be used as a reliable noninvasive method to replace PDSIRM for examining changes in cholesterol absorption. |
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| Publication date | 2004-01 |
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| Terms of use | - The material in this document is covered by the provisions of the Copyright Act, by Canadian laws, policies, regulations and international agreements. Such provisions serve to identify the information source and, in specific instances, to prohibit reproduction of materials without written permission
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| Access condition | |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC publication | This is a non-NRC publication"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC. |
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| NRC number | NRCC 54696 |
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| NPARC number | 9148845 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | bd6d81fb-e1e7-4ffe-86d4-d7492a948038 |
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| Record created | 2009-10-02 |
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| Record modified | 2020-04-17 |
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