| DOI | Resolve DOI: https://doi.org/10.1016/0005-2736(91)90183-9 |
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| Author | Search for: Hübner, Wigand1 ; Search for: Mantsch, Henry H.1 ; Search for: Kates, Morris |
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| Name affiliation | - National Research Council Canada. NRC Steacie Institute for Molecular Sciences
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| Format | Text |
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| Type | Article |
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| Journal title | Biochimica et Biophysica Acta (BBA) - Biomembranes |
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| ISSN | 0005-2736 |
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| Volume | 1066 |
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| Issue | 2 |
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| Pages | 166–174 |
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| Subject | Phospholipid; Cardiolipin; Diphosphatidylglycerol; Deoxycardiolipin; Hydrogen bonding; FTIR |
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| Abstract | Fourier transform infrared (FT-IR) spectroscopy was used to determine whether intramolecular hydrogen bonding between the C-OH and P-OH groups exists in beef heart cardiolipin (CL) or in hydrogenated beef heart cardiolipin (18:0-CL) as compared to the synthetic 2′-deoxy analogue of cardiolipin (16:0-dCL). Such intramolecular hydrogen bonding would provide a structural basis for proton conduction on the molecular level. In aqueous dispersions at 20°C, both 18:0-CL and 16:0-dCL exist in the gel phase as bilayers with gel to liquid-crystalline transitions (Tm) at 61 and 56°C, respectively, whereas the unsaturated CL exists in the non-bilayer (hexagonal II) state. Evidence for intramolecular hydrogen bonding of the C-OH group in aqueous dispersions of 18:0-CL is provided by the large increase in Tm observed on changing the aqueous medium from H2O to D2O but specific hydrogen-bonded C-OH... PO2− species cannot be identified because water molecules also compete for the PO2− binding sites. However, C-OH... PO2− hydrogen bonds can be identified in dry films of the sodium salt of 18:0-CL or in CCl4 solution. In contrast, such hydrogen bonds cannot be formed in the deoxy analogue (16:0-dCL) indicating that the central C-OH group in 18:0-CL could provide a structural basis for proton conduction, involving the phosphate groups. |
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| Publication date | 1991-07-22 |
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| Publisher | Elsevier Science Publishers B.V. |
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| Terms of use | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23001179 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction |
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Record identifier | c2b13947-3504-4a05-bb20-5c370f4f9141 | | Record created | 2017-01-03 |
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| Record modified | 2017-01-03 |
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