| Author | Search for: Incani, V.1; Search for: Danumah, C.1; Search for: Boluk, Y.1 |
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| Affiliation | - National Research Council of Canada. Security and Disruptive Technologies
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| Format | Text, Article |
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| Conference | Nanotech Conference and Expo 2012, June 18-21, 2012, Santa Clara, CA, USA |
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| Subject | Biosensing applications; Biosensor applications; Carbodiimides; Cationic polyethylenimine; Chemical affinities; Enzymes activity; Freeforms; Gold nanoparticles; Immobilized enzyme; Its efficiencies; Metallic nanoparticles; Nanocrystalline cellulose; Natural cellulose fibers; Renewable sources; Surface area; UV-visible; Biosensors; Cellulose derivatives; Exhibitions; Fluorescence spectroscopy; Glucose oxidase; Glucose sensors; Medical nanotechnology; Metal nanoparticles; Nanocomposites; Nanotechnology; Sensors; Enzyme immobilization; Cellulose Derivatives; Enzymes; Glucose; Gold Compounds; Sensors |
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| Abstract | In this work, we describe the use of a composite material made of a renewable source and metallic nanoparticles for biosensing applications. NCC is a product isolated from natural cellulose fibers, which is of approx. 100 nm long and 10 nm wide in size. We augmented the surface area and chemical affinity of NCC by optimally dressing it with gold nanoparticles (AuNP). The deposition of AuNP on NCC was controlled by using cationic Polyethylenimine (PEI). AuNPs were thio-funtionalized prior to enzyme immobilization and the enzyme (glucose oxidase) was conjugated on the composite by carbodiimide (EDC)/NHS coupling. Using UV-visible and fluorescence spectroscopy in the presence of a specific substrate we monitored the activity of the immobilized enzymes, and its efficiency was compared with that of the enzyme in free form. Our results using FT-IR and SEM show that thiol-AuNPs were attached to the surface of NCC. The enzymes activity were measured and quantified using different kit assays and proved that the enzymes were attached to the support and maintained their activity. |
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| Publication date | 2012 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21270285 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | c3c53346-ddd2-4be2-aeac-3b6639aa91ff |
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| Record created | 2014-01-20 |
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| Record modified | 2020-04-21 |
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