| DOI | Resolve DOI: https://doi.org/10.1016/j.jbiotec.2016.05.014 |
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| Author | Search for: Kryachko, Y.1; Search for: Semler, D.; Search for: Vogrinetz, J.; Search for: Lemke, M.; Search for: Links, M. G.1; Search for: McCarthy, E. L.1; Search for: Haug, B.1; Search for: Hemmingsen, S. M.1 |
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| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
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| Format | Text, Article |
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| Subject | interfacial tension; microbially enhanced oil recovery (MEOR); microbial community; cpn60 |
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| Abstract | Microorganisms indigenous to an oil reservoir were grown in media containing either sucrose or proteins in four steel vessels under anoxic conditions at 30 °C and 8.3 MPa for 30 days, to enrich biosurfactant producers. Fermentation of substrate was possible in the protein-containing medium and either fermentation or respiration through reduction of sulfate occurred in the sucrose-containing medium. Growth of microorganisms led to 3.4–5.4-fold surface tension reduction indicating production of biosurfactants in amounts sufficient for enhancement of gas-driven oil recovery. Analysis of sequenced cpn60 amplicons showed that Pseudomonas sp. highly similar to biosurfactant producing P. fluorescens and to Pseudomonas sp. strain TKP predominated, and a bacterium highly similar to biosurfactant producing Bacillus mojavensis was present in vessels. Analysis of 16S rDNA amplicons allowed only genus-level identification of these bacteria. Thus, cpn60-amplicon analysis was a more relevant tool for identification of putative biosurfactant producers than 16S rDNA-amplicon analysis. |
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| Publication date | 2016-05-19 |
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| Publisher | Elsevier |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23001951 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | c4499902-694c-4c25-99b2-374e4a2944c6 |
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| Record created | 2017-06-27 |
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| Record modified | 2020-03-16 |
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