Enrichment and identification of biosurfactant-producing oil field microbiota utilizing electron acceptors other than oxygen and nitrate

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.jbiotec.2016.05.014
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Name affiliation
  1. National Research Council of Canada. Aquatic and Crop Resource Development
FormatText, Article
Journal titleJournal of Biotechnology
ISSN0168-1656
1873-4863
Volume231
Pages9–15
Subjectinterfacial tension; microbially enhanced oil recovery (MEOR); microbial community; cpn60
Abstract
Microorganisms indigenous to an oil reservoir were grown in media containing either sucrose or proteins in four steel vessels under anoxic conditions at 30 °C and 8.3 MPa for 30 days, to enrich biosurfactant producers. Fermentation of substrate was possible in the protein-containing medium and either fermentation or respiration through reduction of sulfate occurred in the sucrose-containing medium. Growth of microorganisms led to 3.4–5.4-fold surface tension reduction indicating production of biosurfactants in amounts sufficient for enhancement of gas-driven oil recovery. Analysis of sequenced cpn60 amplicons showed that Pseudomonas sp. highly similar to biosurfactant producing P. fluorescens and to Pseudomonas sp. strain TKP predominated, and a bacterium highly similar to biosurfactant producing Bacillus mojavensis was present in vessels. Analysis of 16S rDNA amplicons allowed only genus-level identification of these bacteria. Thus, cpn60-amplicon analysis was a more relevant tool for identification of putative biosurfactant producers than 16S rDNA-amplicon analysis.
Publication date
PublisherElsevier
LanguageEnglish
Peer reviewedYes
NPARC number23001951
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