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Viability, cell division and microcallus formation of barley microspores in culture

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF00234694
AuthorSearch for: 1
Affiliation
  1. National Research Council of Canada. NRC Plant Biotechnology Institute
FormatText, Article
Subjectglucose; sugar; sucrose; glycol; cell division
Abstract
Barley microspores were viable when cultured in a sugarless medium. Adding 2g of glucose to 1l of this medium resulted in a significant reduction in the frequency of viable microspores. The frequency of viable microspores was further reduced when 50g of cellobiose, glucose, maltose, melezitose, raffinose or sucrose were added to 1l of the culture medium containing 2g/l glucose. Adding 50g of melibiose, Ficoll, polyethylene glycol (PEG) or a combination 50g each of Ficoll and PEG to 1I of the medium containing 2g/l glucose had very little effect on the viability of the microspores. Up to 66% of the viable microspores were able to divide and many of these developed into microcalli in the basal medium complemented with melibiose, maltose, melezitose, raffinose, Ficoll, PEG or a combination of Ficoll with PEG. Sucrose, cellobiose and glucose added in large quantities inhibited cell division in microspores or destabilized the microspores and only very few of them developed into microcalli. The microcalli in the PEG, Ficoll, Ficoll-PEG and melibiose media were smaller in size than those grown in the melezitose, maltose and raffinose media. Sustained cell division and microcallus formation were observed in a medium with melibiose or maltose as sole source of sugars.
Publication date
PublisherSpringer Nature
In
  • Plant Cell Reports 12-12, no. 7-8 (May 1993).
LanguageEnglish
Peer reviewedYes
IdentifierBF00234694
NRCC-35126
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Record created2023-11-06
Record modified2023-11-06
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