The FRK1 mitogen-activated protein kinase kinase kinase (MAPKKK) from Solanum chacoense is involved in embryo sac and pollen development

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DOI	Resolve DOI: https://doi.org/10.1093/jxb/eru524
Author	Search for: Lafleur, E.; Search for: Kapfer, C.; Search for: Joly, V.; Search for: Liu, Yang; Search for: Tebbji, F.¹; Search for: Daigle, C.; Search for: Gray-mitsumune, M.; Search for: Cappadocia, M.; Search for: Nantel, A.¹; Search for: Matton, D. P.
Name affiliation	National Research Council of Canada. Human Health Therapeutics
Format	Text, Article
Journal title	Journal of Experimental Botany
ISSN	0022-0957 1460-2431
Volume	66
Issue	7
Pages	1833–1843
Abstract	The fertilization-related kinase 1 (ScFRK1), a nuclear-localized mitogen-activated protein kinase kinase kinase (MAPKKK) from the wild potato species Solanum chacoense, belongs to a small group of pMEKKs that do not possess an extended N- or C-terminal regulatory domain. Initially selected based on its highly specific expression profile following fertilization, in situ expression analyses revealed that the ScFRK1 gene is also expressed early on during female gametophyte development in the integument and megaspore mother cell and, later, in the synergid and egg cells of the embryo sac. ScFRK1 mRNAs are also detected in pollen mother cells. Transgenic plants with lower or barely detectable levels of ScFRK1 mRNAs lead to the production of small fruits with severely reduced seed set, resulting from a concomitant decline in the number of normal embryo sacs produced. Megagametogenesis and microgametogenesis were affected, as megaspores did not progress beyond the functional megaspore (FG1) stage and the microspore collapsed around the first pollen mitosis. As for other mutants that affect embryo sac development, pollen tube guidance was severely affected in the ScFRK1 transgenic lines. Gametophyte to sporophyte communication was also affected, as observed from a marked change in the transcriptomic profiles of the sporophytic tissues of the ovule. The ScFRK1 MAPKKK is thus involved in a signalling cascade that regulates both male and female gamete development.
Publication date	2015-04-01
Publisher	Oxford University Press
Language	English
Peer reviewed	Yes
NPARC number	23001681
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Record identifier	c756149d-75b5-4190-b99c-af1fa85c6fd0
Record created	2017-03-17
Record modified	2020-05-30

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Date modified:: 2021-01-27