Specific binding of phenylalanine and tryptophan to β-nicotinamide adenine dinucleotide

DOI	Resolve DOI: https://doi.org/10.1139/v79-367
Author	Search for: Neurohr, Klaus J.¹; Search for: Mantsch, Henry H.¹
Name affiliation	National Research Council Canada
Format	Text
Type	Article
Journal title	Canadian Journal of Chemistry
ISSN	0008-4042 1480-3291
Volume	57
Issue	17
Pages	2297–2301
Abstract	This ¹H nmr study provides the first experimental evidence for specific complex formation between the coenzyme nicotinamide adenine dinucleotide (β-NAD⁺) and the two aromatic amino acids phenylalanine and tryptophan. It is shown that the interaction is of the stacking type and occurs between the aromatic side chains of these amino acids and the adenine moiety of NAD⁺. Binding parameters were obtained from dilution studies, taking into account the self-association of NAD⁺. The apparent microscopic association constants for complex formation of phenylalanine and tryptophan with excess NAD+ in aqueous solutions are 1.35 M⁻¹ and 11.35 M⁻¹, respectively, indicating that the adenine moiety of NAD⁺ can discriminate between phenylalanine and tryptophan by a stronger binding to the latter. The results are discussed with regard to the interaction of NAD⁺ with dehydrogenases.
Publication date	1979-09
Language	English
Peer reviewed	Yes
NRC number	NRCC 17491
NPARC number	23001402
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Record identifier	c789926c-3ae7-497c-946f-f0b43482f7d0
Record created	2017-02-02
Record modified	2020-03-13