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Chirp modulation stimulated Raman scattering microscopy

From National Research Council Canada

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DOIResolve DOI: https://doi.org/10.1364/OE.531274
AuthorSearch for: 1ORCID identifier: https://orcid.org/0000-0003-3334-227X; Search for: 2ORCID identifier: https://orcid.org/0000-0002-8447-3678
Affiliation
  1. National Research Council of Canada. Metrology Research Centre
  2. National Research Council of Canada. Quantum and Nanotechnologies
FunderSearch for: National Research Council of Canada; Search for: Natural Sciences and Engineering Research Council of Canada; Search for: Canada Foundation for Innovation; Search for: Max-Planck-Centre for Extreme and Quantum Photonics
FormatText, Article
Abstract
Coherent Raman microscopy, a rapid, chemical-specific, label-free imaging method, can be plagued by non-Raman background signals. Existing modulation schemes mitigate these but none remove all background signals. Here we demonstrate what we believe to be a novel scheme, chirp modulation stimulated Raman scattering (CM-SRS), based upon modulating uniquely the relative sign of the quadratic phase (linear chirp) of the input lasers. CM-SRS removes all non-Raman signals and is linear in both Raman oscillator strength and concentration. We demonstrate that CM-SRS is highly sensitive, quantitative, and background-free via imaging of traditionally challenging samples and the small molecule pharmacokinetics of single living cells.
Publication date
PublisherOptica Publishing Group
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  • Optics Express 32, no. 18 (13 August 2024): 31297–31310.
LanguageEnglish
Peer reviewedYes
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Record identifierc8e228ff-a82b-4d07-8d56-2767f161e633
Record created2024-09-24
Record modified2024-09-25
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