Form and function: the factors that influence the efficacy of nanomaterials for gene transfer to plants

From National Research Council Canada

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DOI	Resolve DOI: https://doi.org/10.3390/molecules30030446
Author	Search for: Osmani, Zhila¹; Search for: Kulka, Marianna¹ORCID identifier: https://orcid.org/0000-0003-4741-9290
Affiliation	National Research Council Canada. Quantum and Nanotechnologies
Funder	Search for: NSERC Alliance; Search for: National Research Council Canada; Search for: Saskatchewan Canola Development Commission; Search for: Western Grains Research Foundation
Format	Text, Article
Subject	nanoparticles; gene delivery; plant biotechnology; plant transformation; crop improvement; biocompatibility
Abstract	Nanoparticle (NP)-mediated gene delivery offers a promising alternative to traditional methods in plant biotechnology, facilitating genetic transformations with enhanced precision and efficiency. This review discusses key factors influencing NP efficacy, including plant cell wall composition, DNA/NP ratios, exposure time, cargo loading, and post-transformation assessments. We explore the challenges of NP cytotoxicity, transformation efficiency, and regeneration while addressing environmental impacts and regulatory considerations. We emphasize the potential for stimulus-responsive NPs and scalable delivery methods to optimize gene editing in agriculture.
Publication date	2025-01-21
Publisher	MDPI
Licence	Creative Commons, Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
In	Molecules 30, no. 3, 446 (21 January 2025).
Language	English
Peer reviewed	Yes
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Record identifier	c9a3f961-7ee2-4d7b-b75d-2e0e282c4ac4
Record created	2025-05-15
Record modified	2025-11-03

Date modified:: 2026-01-20