| Download | - View final version: Pentavalent single-domain antibodies reduce Campylobacter jejuni motility and colonization in chickens (PDF, 1.4 MiB)
|
|---|
| DOI | Resolve DOI: https://doi.org/10.1371/journal.pone.0083928 |
|---|
| Author | Search for: Riazi, Ali; Search for: Strong, Philippa C. R.1; Search for: Coleman, Russell1; Search for: Chen, Wangxue1; Search for: Hirama, Tomoko2; Search for: van Faassen, Henk1; Search for: Henry, Matthew; Search for: Logan, Susan M.1; Search for: Szymanski, Christine M.; Search for: MacKenzie, Roger1; Search for: Ghahroudi, Mehdi Arbabi1 |
|---|
| Affiliation | - National Research Council of Canada. Human Health Therapeutics
- National Research Council of Canada. NRC Institute for Biological Sciences
|
|---|
| Format | Text, Article |
|---|
| Abstract | Campylobacter jejuni is the leading cause of bacterial foodborne illness in the world, with symptoms ranging from acute diarrhea to severe neurological disorders. Contaminated poultry meat is a major source of C. jejuni infection, and therefore, strategies to reduce this organism in poultry, are expected to reduce the incidence of Campylobacter-associated diseases. We have investigated whether oral administration of C. jejuni-specific single-domain antibodies would reduce bacterial colonization levels in chickens. Llama single-domain antibodies specific for C. jejuni were isolated from a phage display library generated from the heavy chain IgG variable domain repertoire of a llama immunized with C. jejuni flagella. Two flagella-specific single-domain antibodies were pentamerized to yield high avidity antibodies capable of multivalent binding to the target antigen. When administered orally to C. jejuni-infected two-day old chicks, the pentabodies significantly reduced C. jejuni colonization in the ceca. In vitro, the motility of the bacteria was also reduced in the presence of the flagella-specific pentabodies, suggesting the mechanism of action is through either direct interference with flagellar motility or antibody-mediated aggregation. Fluorescent microscopy and Western blot analyses revealed specific binding of the anti-flagella pentabodies to the C. jejuni flagellin. |
|---|
| Publication date | 2013-12-31 |
|---|
| Publisher | PLOS |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NPARC number | 23000585 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | c9f638d2-25f9-438f-b2d9-52b5cfa4d3a6 |
|---|
| Record created | 2016-08-03 |
|---|
| Record modified | 2021-09-20 |
|---|
