A functional genomics method for assaying gene function in phytopathogenic fungi through host-induced gene silencing mediated by agroinfiltration

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/978-1-4939-2453-0_13
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Name affiliation
  1. National Research Council of Canada. Aquatic and Crop Resource Development
FormatText, Book chapter
Book titlePlant Gene Silencing: Methods and Protocols
ISBN9781493924530
9781493924523
Pages179–189
Abstract
With the rapid growth of genomic information, there is an increasing demand for efficient analysis tools to study the function of predicted genes coded in genomes. Agroinfiltration, the delivery of gene constructs into plant cells by Agrobacterium tumefaciens infiltrated into leaves, is one such versatile, simple, and rapid technique that is increasingly used for transient gene expression assay in plants. In this chapter, we focus on the use of agroinfiltration as a functional genomics research tool in molecular plant pathology. Specifically, we describe in detail its use in expressing phytopathogenic fungal gene sequences in a host plant to induce RNA silencing of corresponding genes inside the pathogen, a method which has been termed host-induced gene silencing (HIGS). We target the fungal pathogen Puccinia triticina which causes leaf rust on its wheat host, but the method is applicable to a variety of pathosystems.
Publication date
PublisherSpringer International Publishing
LanguageEnglish
Peer reviewedYes
NPARC number21277408
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Record created2016-03-09
Record modified2020-06-11
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