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Anatoxins in cyanobacterial mat field samples from Atlantic Canada by direct analysis in real time–high resolution tandem mass spectrometry

From National Research Council Canada

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DOIResolve DOI: https://doi.org/10.5281/zenodo.7035072
AuthorSearch for: 1ORCID identifier: https://orcid.org/0000-0002-5680-2112; Search for: ORCID identifier: https://orcid.org/0000-0002-1756-4297; Search for: ; Search for: ; Search for: 1; Search for: 1ORCID identifier: https://orcid.org/0000-0002-7746-8432
Affiliation
  1. National Research Council of Canada. Metrology Research Centre
FormatText, Article
ConferenceHarmful Algae 2019 - International Conference on Harmful Algae (ICHA 2021), October 10-15, 2021, La Paz, Mexico [Held Virtually]
SubjectCyanotoxins; Phormidium; Microcoleus; ambient ionization
Abstract
Toxic freshwater benthic cyanobacterial mats are increasingly linked to animal fatalities worldwide, often due to the production of high concentrations of anatoxins (ATXs). Considering the high spatial and temporal variability of mat occurrence and concentrations of ATXs, suitable analytical methods are required to enable large-scale field studies in these systems. Here, a rapid, direct analysis in real time–high resolution tandem mass spectrometry (DART–HRMS/MS) method for analysis of anatoxin-a, homoanatoxin-a, and dihydroanatoxin-a including a simple sample preparation procedure and isotope dilution calibration, is investigated to address this challenge. The developed method was applied to three sets of cyanobacterial mat samples collected in Atlantic Canada in the summers of 2018-2020. Samples from all sets showed positive detection of ATXs ranging from <100 ng mL⁻¹ to > 70,000 ng mL⁻¹ in mat lysates. One large mat sample collected from the Wolastoq (Saint John River) in New Brunswick, Canada, was used to demonstrate the application of DART–HRMS/MS to study the intra-mat variability of ATXs. This initial work showed the method could be successfully used for the analysis of ATXs in field samples, which will be useful in ongoing studies of this emerging issue in Atlantic Canada and beyond.
Publication date
PublisherZenodo
International Society for the Study of Harmful Algae (ISSHA)
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  • Proceedings of the 19th International Conference on Harmful Algae (ICHA 2021) (21 September 2022): 151–156.
LanguageEnglish
Peer reviewedYes
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Record identifiercc222bdf-9356-44ef-a9b4-486ee440886d
Record created2024-01-15
Record modified2024-01-15
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