| Author | Search for: Altman, Eleonora; Search for: Fernandez, H.; Search for: Chandan, Vandana; Search for: Harrison, B.; Search for: Schuster, M.; Search for: Rademacher, L.; Search for: Toledo, C. |
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| Format | Text, Article |
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| Subject | adolescent; adult; aged; aged, 80 and over; antibody; antigen; antigens, CD15; blood; blood group; Canada; Chile; epidemiology; epitope; expression; female; gastrointestinal diseases; gene expression regulation, bacterial; genetics; helicobacter infections; helicobacter pylori; immunology; Lewis; Lewis blood group system; Lipopolysaccharide; LPS; male; metabolism; microbiology; middle aged; oligosaccharide; physiology; selective; strain; structure; system |
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| Abstract | Previous studies have shown that the LPS of Helicobacter pylori isolated from North American and European hosts predominantly expresses type 2 Lewis x (Le(x)) and Le(y) epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Le(a) and Le(b) structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by H. pylori isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of H. pylori isolates were assessed by gel electrophoresis and Western blotting. Expression of Le(x) and/or Le(y) epitopes was confirmed in 32/38 isolates (84 %), whilst 9/38 isolates (24 %) expressed type 1 Le(b) blood group determinants, in addition to type 2 Le(x) and Le(y) structures. Six strains (16 %) were non-typeable. The majority of H. pylori strains examined were CagA-positive (83.3 %) |
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| Publication date | 2008-05 |
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| In | |
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| Language | English |
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| NRC number | ALTMAN2008 |
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| NPARC number | 9369974 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | cf589149-4fd8-4e98-89ba-fb88600f219f |
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| Record created | 2009-07-10 |
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| Record modified | 2020-04-15 |
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