| Download | - View final version: Fly ash based robust biocatalyst generation: a sustainable strategy towards enhanced green biosurfactant production and waste utilization (PDF, 1.2 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1039/c9ra02784j |
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| Author | Search for: Zhu, Zhiwen; Search for: Zhang, Baiyu; Search for: Chen, Bing; Search for: Ling, Jingjing; Search for: Cai, Qinghong1; Search for: Husain, Tahir |
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| Affiliation | - National Research Council of Canada. Energy, Mining and Environment
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| Format | Text, Article |
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| Abstract | Biosurfactants have been well recognized as an environmentally friendly alternative to chemical surfactants. However, their production remains challenging due to low productivity, short-term microbe stability and the potentially toxic by-products generated in the growth media. To overcome these challenges, the emerging biofilm-based biosynthesis was investigated in this study. A fresh insight into the biosynthesis process was provided through using waste fly ash as a carrier material. The biofilm produced by biosurfactant producer B. subtilis N3-1P attached onto the surface of fly ash acted as a robust and effective biocatalyst. Zeta potential analysis and scanning electron microscope (SEM) characterization were conducted to help unravel the biocatalyst formation. High-value biosurfactant products were then produced in an efficient and sustainable manner. Stimulation by a fly ash assisted biocatalyst on biosurfactant production was confirmed. The biosurfactant yield was boosted over ten times after 24 hours, at a fly ash dosage of 0.5%. The highest biosurfactant yield was achieved after five days, with a final productivity of 305 critical micelle dilution. The underlying mechanism of fly ash assisted biosurfactant production was tracked through it exerting an effect on the quorum sensing system. Fourier-transform infrared (FTIR) spectroscopy and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis demonstrated that the final biosurfactant product belonged to the lipopeptides. This research output is expected to accelerate the development of more effective bioreactors, and make a continuous contribution to high-value product generation and waste reduction. |
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| Publication date | 2019 |
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| Publisher | Royal Society of Chemistry |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | d03f691b-a184-4dea-97df-6edb534d541c |
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| Record created | 2020-11-25 |
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| Record modified | 2020-12-14 |
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