| DOI | Resolve DOI: https://doi.org/10.1111/j.1574-6968.1985.tb00984.x |
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| Author | Search for: Louise M. Nelson1; Search for: Grosskopf, E.; Search for: Tichy, H.V.; Search for: Lotz, W. |
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| Affiliation | - National Research Council Canada. NRC Plant Biotechnology Institute
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| Format | Text, Article |
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| Subject | uptake hydrogenase; hybridization; plasmid; DNA homology |
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| Abstract | 17 Strains of Rhizobium leguminosarum which varied in phenotypic expression of uptake hydrogenase (Hup) were analyzed for the presence of hup-specific DNA sequences using hybridization probes constructed from hup-specific DNA cloned from Rhizobium japonicum (PHVT100) and R. leguminosarum (PHVT109 and PHVT115). 2 Hup− strains and 3 strains with trace amounts of Hup activity showed no hybridization. 10 Hup⁺ strains hybridized with all 3 probes, indicating that hup-specific DNA sequences are conserved in these strains. 2 Hup⁺ strains did not hybridize with any of the probes. These strains may possess significantly different DNA sequences determining the Hup phenotype. |
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| Publication date | 1985-10-01 |
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| Publisher | Oxford University Press |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC-24521 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | d1b12b99-33a2-4315-8869-f046684f9633 |
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| Record created | 2023-06-28 |
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| Record modified | 2023-06-29 |
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