| DOI | Resolve DOI: https://doi.org/10.1111/j.1574-6968.1985.tb00984.x |
|---|
| Author | Search for: Louise M. Nelson1; Search for: Grosskopf, E.; Search for: Tichy, H.V.; Search for: Lotz, W. |
|---|
| Affiliation | - National Research Council of Canada. NRC Plant Biotechnology Institute
|
|---|
| Format | Text, Article |
|---|
| Subject | uptake hydrogenase; hybridization; plasmid; DNA homology |
|---|
| Abstract | 17 Strains of Rhizobium leguminosarum which varied in phenotypic expression of uptake hydrogenase (Hup) were analyzed for the presence of hup-specific DNA sequences using hybridization probes constructed from hup-specific DNA cloned from Rhizobium japonicum (PHVT100) and R. leguminosarum (PHVT109 and PHVT115). 2 Hup− strains and 3 strains with trace amounts of Hup activity showed no hybridization. 10 Hup⁺ strains hybridized with all 3 probes, indicating that hup-specific DNA sequences are conserved in these strains. 2 Hup⁺ strains did not hybridize with any of the probes. These strains may possess significantly different DNA sequences determining the Hup phenotype. |
|---|
| Publication date | 1985-10-01 |
|---|
| Publisher | Oxford University Press |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NRC number | NRCC-24521 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | d1b12b99-33a2-4315-8869-f046684f9633 |
|---|
| Record created | 2023-06-28 |
|---|
| Record modified | 2023-06-29 |
|---|
