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The effect of different promoter-sequences on transient expression of gus reporter gene in cultured barley (Hordeum vulgare L.) cells

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF00236096
AuthorSearch for: 1; Search for: 1; Search for: 1; Search for: 1; Search for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council Canada. NRC Plant Biotechnology Institute
FormatText, Article
Subjectparticle bombardment; Hordeum vulgare L.; promoter screening; transient expression; gus; cereal transformation
Abstract
The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcohol dehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intronl along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression of the E.coli β-glucuronidase (gus) reporter gene in cultured barley (Hordeum vulgare L) cells. The plasmids, carrying the respective promoterintron combinations to drive the gus fused to nopaline synthase (nos) terminator, were introduced into cultured barley cells using a particle gun. The rice Act1 promoter with its first intron gave the highest expression of all promoter intron combinations studied. This was followed by the E35S promoter and no significant differences were observed between the other two promoters tested. The rice actin promoter is now being used to drive selectable marker genes to obtain stably transformed cereal cells.
Publication date
PublisherSpringer Nature
In
  • Plant Cell Reports 12, no. 9 (July 1993).
LanguageEnglish
Peer reviewedYes
IdentifierBF00236096
NRCC-36482
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Record created2023-11-06
Record modified2023-11-06
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