| DOI | Resolve DOI: https://doi.org/10.1074/jbc.M705424200 |
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| Author | Search for: Paris, Sébastien1; Search for: Burlacu, Alina1; Search for: Durocher, Yves1 |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | biotechnology; cells; endocytosis; gene expression; incomplet; proteoglycans; tail; transfection; polyethyleneimine; clustering |
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| Abstract | Polyethylenimines (PEIs) are efficient non-viral vectors for gene transfer. Heparan sulphate proteoglycans have been proposed to be the cell surface receptors for PEI-DNA complexes (polyplexes). Here, we investigated if syndecan-1 (SDC1) and syndecan-2 (SDC2) may be involved in PEI-mediated transfection. Following addition of polyplexes to HEK293 cells, GFP-tagged SDCs rapidly formed clusters with PEI that were dependent of lipid rafts integrity. However, while SDC1 overexpression slightly enhanced PEImediated gene expression, SDC2 dramatically inhibited it. Confocal microscopy analysis showed that SDC1:polyplex endocytosis occurs within minutes after addition of polyplexes while that of SDC2:polyplexes takes hours. Expression of SDC1 cytoplasmic deletion mutants revealed that the SDC1 cytoplasmic tail is required for gene expression, but not for clustering or endocytosis, while overexpression of SDC1/SDC2 chimeras showed that SDC2 ectodomain is responsible for the inhibitory effect on gene transfer. Taken together, this study provides evidence that SDCs may have opposing effects on PEI-mediated transfection. |
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| Publication date | 2008-03-21 |
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| Publisher | American Society for Biochemistry and Molecular Biology |
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| In | |
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| Note | DA - 20080124IS - 0021-9258 (Print)LA - ENGPT - JOURNAL ARTICLE |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 47810
NRC-BRI-47810 |
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| NPARC number | 8925865 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | d3e4fdc5-a460-4644-b40c-5de321a324a3 |
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| Record created | 2009-04-23 |
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| Record modified | 2020-04-15 |
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