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Measurement of macrophage-mediated killing of intracellular bacteria, including Francisella and Mycobacteria

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1002/0471142735.im1425s93
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Affiliation
  1. National Research Council of Canada. NRC Institute for Biological Sciences
FormatText, Article
SubjectFrancisella; Mycobacteria; intracellular bacteria; macrophage; cytokine; intracellular killing; T lymphocytes
Abstract
Macrophages activated by T cell cytokines are a critical defense mechanism against intracellular bacterial pathogens. This unit presents two general methods for assessing the capacity of mouse macrophages, activated with either soluble cytokines or whole immune T lymphocytes, to control or reduce numbers of intracellular bacteria residing within them. “Measurement of killing” is inferred from a reduction in the number of colony-forming units (cfu) of bacteria at the end of a culture period, compared to the input numbers of cfu at initiation of culture, to the peak numbers of cfu measured during culture, or to a control group in which killing is expected to be poor.
Publication date
In
  • Current Protocols in Immunology, no. Suppl. 93, 14.25 (1 April 2011): 14.25.1–14.25.13.
LanguageEnglish
Peer reviewedYes
NPARC number19230297
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Record identifierd49a80ec-f849-4114-a5eb-4fa4b59db687
Record created2012-03-08
Record modified2020-04-21
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