Overexpression of selenocysteine methyltransferase in Arabidopsis and Indian mustard increases selenium tolerance and accumulation

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1104/pp.103.026989
Author	Search for: Leduc, D. L.; Search for: Tarun, Alice S.; Search for: Montes-Bayon, Maria; Search for: Meija, Juris ORCID identifier: https://orcid.org/0000-0002-3349-5535; Search for: Malit, Michele F.; Search for: Wu, Carol P.; Search for: AbdelSamie, Manal; Search for: Chiang, Chih-Yuan Chiang; Search for: Tagmount, Abderrhamane; Search for: DeSouza, Mark; Search for: Neuhierl, Bernhard; Search for: Böck, August; Search for: Caruso, Joseph; Search for: Terry, Norman
Format	Text, Article
Abstract	A major goal of phytoremediation is to transform fast-growing plants with genes from plant species that hyperaccumulate toxic trace elements. We overexpressed the gene encoding selenocysteine methyltransferase (SMT) from the selenium (Se) hyperaccumulator Astragalus bisulcatus in Arabidopsis and Indian mustard (Brassica juncea). SMT detoxifies selenocysteine by methylating it to methylselenocysteine, a nonprotein amino acid, thereby diminishing the toxic misincorporation of Se into protein. Our Indian mustard transgenic plants accumulated more Se in the form of methylselenocysteine than the wild type. SMT transgenic seedlings tolerated Se, particularly selenite, significantly better than the wild type, producing 3- to 7-fold greater biomass and 3-fold longer root lengths. Moreover, SMT plants had significantly increased Se accumulation and volatilization. This is the first study, to our knowledge, in which a fast-growing plant was genetically engineered to overexpress a gene from a hyperaccumulator in order to increase phytoremediation potential.
Publication date	2003-12-11
Publisher	American Society of Plant Biologists
In	Plant Physiology 135, no. 1: 377–383.
Language	English
Peer reviewed	Yes
NRC publication	This is a non-NRC publication "Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.
NPARC number	23001835
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Record identifier	d761e52e-22de-47e9-a3b9-5e5d760bebc6
Record created	2017-04-18
Record modified	2020-04-02

Date modified:: 2024-12-04