Overexpression of selenocysteine methyltransferase in Arabidopsis and Indian mustard increases selenium tolerance and accumulation

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1104/pp.103.026989
AuthorSearch for: ; Search for: ; Search for: ; Search for: ORCID identifier: https://orcid.org/0000-0002-3349-5535; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
FormatText, Article
Journal titlePlant Physiology
ISSN0032-0889
1532-2548
Volume135
Issue1
Pages377–383
Abstract
A major goal of phytoremediation is to transform fast-growing plants with genes from plant species that hyperaccumulate toxic trace elements. We overexpressed the gene encoding selenocysteine methyltransferase (SMT) from the selenium (Se) hyperaccumulator Astragalus bisulcatus in Arabidopsis and Indian mustard (Brassica juncea). SMT detoxifies selenocysteine by methylating it to methylselenocysteine, a nonprotein amino acid, thereby diminishing the toxic misincorporation of Se into protein. Our Indian mustard transgenic plants accumulated more Se in the form of methylselenocysteine than the wild type. SMT transgenic seedlings tolerated Se, particularly selenite, significantly better than the wild type, producing 3- to 7-fold greater biomass and 3-fold longer root lengths. Moreover, SMT plants had significantly increased Se accumulation and volatilization. This is the first study, to our knowledge, in which a fast-growing plant was genetically engineered to overexpress a gene from a hyperaccumulator in order to increase phytoremediation potential.
Publication date
PublisherAmerican Society of Plant Biologists
LanguageEnglish
Peer reviewedYes
NRC publication
This is a non-NRC publication

"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.

NPARC number23001835
Export citationExport as RIS
Report a correctionReport a correction
Record identifierd761e52e-22de-47e9-a3b9-5e5d760bebc6
Record created2017-04-18
Record modified2020-04-02
Report a problem on this page
Date modified: