| Download | - View accepted manuscript: Multilabeling biomolecules at a single site. 1. Synthesis and characterization of a dendritic label for electrochemiluminescence assays (PDF, 808 KiB)
|
|---|
| DOI | Resolve DOI: https://doi.org/10.1021/ac034664d |
|---|
| Author | Search for: Zhou, Ming1; Search for: Roovers, Jacques2; Search for: Robertson, Gilles P.2; Search for: Grover, Chander P.1 |
|---|
| Affiliation | - National Research Council of Canada. NRC Institute for National Measurement Standards
- National Research Council of Canada. NRC Institute for Chemical Process and Environmental Technology
|
|---|
| Format | Text, Article |
|---|
| Abstract | Ultrasensitive bioanalytical assays are of great value for early detection of human diseases and pathogens. The sensitivities of immunoassays and DNA probing can be enhanced by multilabeling the biorecognition partner used for affinity-based assays. However, the bioreactivity of biomolecules is affected by a high degree of multilabeling at multiple functional sites. It is proposed that dendritic scaffoldings be used to link multiple signal-generating units to a single site with potentially minimum impact on the bioaffinity. A prototype label, a zeroth-generation dendron, bearing three [Ru(bpy)₃]²⁺ units for electrochemiluminescence (ECL) assays was synthesized and characterized preliminarily by spectroscopic, electrochemical, and ECL methods. No evidence of interaction between the neighboring [Ru(bpy)₃]²⁺ units in the label molecule was found from these characterizations. Both the photoluminescence and ECL of the prototype label have features very similar to those of mononuclear [Ru- (bpy)₃]²⁺ compounds. Labeling a model protein with a triad of [Ru(bpy)₃]²⁺ at one NH₂ position was demonstrated. The results reported here provide support to applying the proposed multilabeling strategy to affinitybased bioanalytical assays. |
|---|
| Publication date | 2003-12 |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NRC number | NRCC 51678 |
|---|
| NPARC number | 14542472 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | d795fddf-c5ee-4f0a-a89f-f0f12ff122f8 |
|---|
| Record created | 2010-03-01 |
|---|
| Record modified | 2020-04-02 |
|---|
