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| DOI | Resolve DOI: https://doi.org/10.1016/j.mcpro.2023.100645 |
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| Author | Search for: Chen, Rui1ORCID identifier: https://orcid.org/0000-0003-3149-9492; Search for: Fulton, Kelly M.1; Search for: Tran, Anh1ORCID identifier: https://orcid.org/0000-0003-0644-6014; Search for: Duque, Diana1; Search for: Kovalchik, Kevin; Search for: Caron, Etienne; Search for: Twine, Susan M.1; Search for: Li, Jianjun1 |
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| Affiliation | - National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Article |
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| Abstract | We present an integrated immunopeptidomics and proteomics study of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection to comprehensively decipher the changes in host cells in response to viral infection. Immunopeptidomics analysis identified viral antigens presented by host cells through both class I and class II MHC system for recognition by the adaptive immune system. The host proteome changes were characterized by quantitative proteomics and glycoproteomics and from these data, the activation of toll-like receptor 3–interferon pathway was identified. Glycosylation analysis of human leukocyte antigen (HLA) proteins from the elution and flow-through of immunoprecipitation revealed that SARS-CoV-2 infection changed the glycosylation pattern of certain HLA alleles with different HLA alleles, showing distinct dynamic changes in relative abundance. The difference in the glycosylation and abundance of HLA alleles changed the number of strong binding antigens each allele presented, suggesting the impact of SARS-CoV-2 infection on antigen presentation is allele-specific. These results could be further exploited to explain the imbalanced response from innate and adaptive immune system in coronavirus disease 2019 cases, which would be helpful for the development of therapeutics and vaccine for coronavirus disease 2019 and preparation for future pandemic. |
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| Publication date | 2023-09-13 |
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| Publisher | Elsevier |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | S1535947623001561 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | d8ec2b10-a3db-4cb8-91a7-aedf0bf05877 |
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| Record created | 2024-06-19 |
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| Record modified | 2024-06-19 |
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