| Download | - View accepted manuscript: A simple mathematical model for electric cell-substrate impedance sensing with extended applications (PDF, 836 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1016/j.bios.2009.12.025 |
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| Author | Search for: Xiao, Caide; Search for: Luong, John H.T.1 |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | Electric cell-substrate impedance sensing; Mathematical model; L-Cysteine; Ferri/ferrocyanide; Bacteria; Doubling time; Antibiotics resistance |
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| Abstract | This paper presents a simple mathematical model to predict the impedance data acquired by electric cell-substrate impedance sensing (ECIS) at frequencies between 25 Hz and 60 kHz. With this model, the equivalent resistance (R) and capacitance (C) of biological samples adhered on gold surfaces could be more precisely measured at 4 kHz. ECIS applications were extended for real-time monitoring of living bacteria cultivated in Luria Bertani (LB) culture medium by two different approaches. In the former, we used a ferri/ferrocyanide redox couple in LB medium as an indicator for bacterial multiplication. Because the redox couple was toxic to some bacteria, we developed a second approach, in which L-cysteine self-assembled monolayers (SAM) on gold electrodes were used to detect living bacteria. The L-cysteine SAM could also be detected by ECIS. Unlike traditional impedance microbiological methods which need special culture media with low ions, our procedures significantly enhanced signal/noise ratios so bacteria could be detected in general purpose culture media. It was easy and convenient to obtain bacterial doubling times and evaluate the resistance of bacteria to antibiotics from ECIS spectra. |
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| Publication date | 2010 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 50007 |
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| NPARC number | 14758187 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | d9eb686d-9e9e-463e-95df-bd1cd9561b1b |
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| Record created | 2010-06-02 |
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| Record modified | 2020-04-17 |
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