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Efficiency of cationic rosette nanotubes for siRNA delivery

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1557/opl.2011.435
AuthorSearch for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. National Institute for Nanotechnology
FormatText, Article
Conference2010 MRS Fall Meeting: Symposium QQ: Nanofunctional Materials, Nanostructures and Nanodevices for Biomedical Applications II, November 29 - December 3, 2010, Boston, MA, USA
SubjectAgarose gel; Binding capacities; Cell toxicity; Functionalizations; Gel-retardation assay; Guanine cytosines; Human cell lines; l-Lysine; Loading efficiency; Mole ratio; siRNA delivery; Spherical structures; Supramolecular assemblies; Amino acids; Cell culture; Cytotoxicity; Medical applications; Nanotubes
Abstract
Cationic rosette nanotubes (RNTs) were generated by functionalization of self-complementary twin guanine-cytosine (G∧C) motifs with up to 15 L-lysine residues (Kn.T, n = 1-15). siRNA binding capacity was determined by gel retardation assay on agarose gel. Up to K5.T, siRNA complexation was a function of oligolysine-chain length and mole ratio of Kn.T. At higher Kn.T, local cationic density employed by supramolecular assembly emerged as a contributor to siRNA complexation. We have shown that no effective siRNA binding was achieved with equivalent mole ratios of corresponding oligolysine peptides (not conjugated to the G∧C motif). With K12.T, siRNA complexation gave spherical structures in the range of 200 nm, which was internalized and retained by human cell lines without noticeable cytotoxicity. In this report, we demonstrate for the first time the capacity of the RNTs as siRNA carriers that can be tailored to achieve maximum siRNA loading efficiency without carrier-associated cell toxicity. We anticipate these cationic RNTs to be effective in the delivery of biologically-functional siRNA. © 2011 Materials Research Society.
Publication date
In
  • Nanofunctional materials, nanostructures and nanodevices for biomedical applications II (2 March 2011): 41–46.
Series
  • Materials Research Society Symposium Proceedings 1316.
LanguageEnglish
Peer reviewedYes
NPARC number21271949
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Record modified2020-04-21
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