| DOI | Resolve DOI: https://doi.org/10.1007/BF02055792 |
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| Author | Search for: Fowke, L. C.1; Search for: Bech-Hansen, C. W.1; Search for: Gamborg, O. L.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Subject | cell wall; plant cell; cell division; microscopic observation; cell regeneration |
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| Abstract | Protoplasts of Ammi visnaga initiated cell wall formation within 2 days in culture; after 13 days the new cells were enclosed by a cell wall similar to the walls on the original cultured cells. Budding occurred in protoplasts with little or no detectable cell wall. No evidence was obtained for direct participation of any organelle in cell wall formation. The cytoplasm of regenerating cells contained numerous organelles and appeared typical of actively growing plant cells; they were easily distinguished from degenerate cells and protoplasts. While coated vesicles were common, spiny vesicles occurred in only a few cells. Sustained cell division yielded multicellular aggregates. Multinucleate protoplasts, formed by spontaneous fusion, did not divide; some of them contained annulate lamellae with few pore complexes. |
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| Publication date | 1974-03 |
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| Publisher | Springer Nature |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | BF02055792 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | db987e5c-eb69-44d6-ab39-85cff146b016 |
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| Record created | 2023-07-06 |
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| Record modified | 2023-07-11 |
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