A new subfamily of short bacterial adenylate kinases with the Mycobacteriumtuberculosis enzyme as a model : a predictive and experimental study

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1002/(SICI)1097-0134(19990801)36:2<238::AID-PROT9>3.0.CO;2-K
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Name affiliation
  1. National Research Council of Canada. NRC Institute for Biodiagnostics
FormatText, Article
Journal titleProteins: Structure, Function, and Bioinformatics
ISSN1097-0134
Volume36
Issue2
Pages238–248
Subjectadenylate kinase; short variant; Mycobacterium tuberculosis; NMR spectroscopy; infrared spectroscopy
Abstract
The adk gene from Mycobacterium tuberculosis codes for an enzyme of 181 amino acids. A sequence comparison with 52 different forms of adenylate kinases (AK) suggests that the enzyme from M. tuberculosis belongs to a new subfamily of “short” bacterial AKs. The recombinant protein, overexpressed in Escherichia coli, exhibits a low catalytic activity and an unexpectedly high thermal stability (Tm = 64.8°C). Based on various spectroscopic data, on the known three-dimensional structure of the AK from E. coli and on secondary structure predictions for various sequenced AKs, we propose a structural model for AK from M. tuberculosis (AKmt).
Publication date
PublisherWiley-Liss, Inc.
LanguageEnglish
Peer reviewedYes
NPARC number23000809
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