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Validation of a high-performance liquid chromatographic assay for the quantification of Reovirus particles type 3

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.jpba.2007.06.025
AuthorSearch for: 1; Search for: 1; Search for: 1; Search for: 1; Search for: ; Search for: 1
Name affiliation
  1. National Research Council of Canada. NRC Biotechnology Research Institute
FormatText, Article
Subjectreovirus; oncolytics; HPLC; quantification; validation
Abstract
An anion exhange high-performance liquid chromatography (HPLC) method for the quantification of human Reovirus type 3 particles was validated according to the performance criteria of precision, specificity, linearity of calibration and working range, limits of detection and quantification, accuracy and recovery. Samples taken at various stages of Reovirus purification were used for the validation of the method. The method was specific for Reovirus which eluted around 9.8 min without interference from any other component in the sample. Reovirus can be detected between 0.32E+12 and 2.10E12 VP/mL by the proposed method that has the correlation coefficient of linearity equal to 0.9974 and the slope of linearity equal to 5,74E-07 area units/(VP mL).
Publication date
In
  • Journal of Pharmaceutical and Biomedical Analysis 45, no. 3: 417–421.
LanguageEnglish
Peer reviewedYes
NRC numberNRC-BRI-47812
NPARC number3539542
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Record identifiere0665e93-5428-46d7-822d-e71bd126ca08
Record created2009-03-01
Record modified2020-05-10
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